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cellbrite green stain  (Biotium)


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    Biotium cellbrite green stain
    Cell separation defects lead to phagocytosis of larger clumps. RAW264.7 macrophages were co-incubated with UV-inactivated C. albicans for 2 h and fixed with 4% formaldehyde. Macrophages were stained with <t>CellBrite</t> Green, and C. albicans cells were stained with CFW. ( A ) Confocal microscopy images of macrophages co-incubated with wild type, eng1Δ/Δ, fgr41Δ/Δ, fgr41Δ/ΔENG1 oe , or eng1Δ/Δfgr41Δ/Δ strains. Scale bar = 25 µm. ( B–D ) Quantification of panel A. A total of 40 images were analyzed for each strain. C. albicans cells were counted as being phagocytosed if they appeared internal to the macrophage and/or caused a gap in green fluorescence in the macrophage. ( B ) Percentages of macrophages containing each number of C. albicans cells in panel A. n = the total number of macrophages with at least one C. albicans cell inside them. Graphs including macrophages that had not phagocytosed any C. albicans are found in . ( C ) The number of C. albicans cells inside each macrophage for each strain from panel A. Each point represents a macrophage that had phagocytosed at least one C. albicans cell. The number of macrophages represented in each bar corresponds to the n in panel B. **** P <0.0001, *** P < 0.001, by one-way ANOVA, ns = not significant. ( D ) The wild type and eng1Δ/Δ bars from panel C were analyzed in isolation, which revealed a significant difference (* P < 0.05, by Welch’s t -test).
    Cellbrite Green Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 96/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellbrite green stain/product/Biotium
    Average 96 stars, based on 126 article reviews
    cellbrite green stain - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "Loss of Fgr41 in Candida albicans attenuates virulence and increases proinflammatory immune responses in a manner that is dependent on β(1,3)-glucan but not dectin-1"

    Article Title: Loss of Fgr41 in Candida albicans attenuates virulence and increases proinflammatory immune responses in a manner that is dependent on β(1,3)-glucan but not dectin-1

    Journal: Infection and Immunity

    doi: 10.1128/iai.00523-25

    Cell separation defects lead to phagocytosis of larger clumps. RAW264.7 macrophages were co-incubated with UV-inactivated C. albicans for 2 h and fixed with 4% formaldehyde. Macrophages were stained with CellBrite Green, and C. albicans cells were stained with CFW. ( A ) Confocal microscopy images of macrophages co-incubated with wild type, eng1Δ/Δ, fgr41Δ/Δ, fgr41Δ/ΔENG1 oe , or eng1Δ/Δfgr41Δ/Δ strains. Scale bar = 25 µm. ( B–D ) Quantification of panel A. A total of 40 images were analyzed for each strain. C. albicans cells were counted as being phagocytosed if they appeared internal to the macrophage and/or caused a gap in green fluorescence in the macrophage. ( B ) Percentages of macrophages containing each number of C. albicans cells in panel A. n = the total number of macrophages with at least one C. albicans cell inside them. Graphs including macrophages that had not phagocytosed any C. albicans are found in . ( C ) The number of C. albicans cells inside each macrophage for each strain from panel A. Each point represents a macrophage that had phagocytosed at least one C. albicans cell. The number of macrophages represented in each bar corresponds to the n in panel B. **** P <0.0001, *** P < 0.001, by one-way ANOVA, ns = not significant. ( D ) The wild type and eng1Δ/Δ bars from panel C were analyzed in isolation, which revealed a significant difference (* P < 0.05, by Welch’s t -test).
    Figure Legend Snippet: Cell separation defects lead to phagocytosis of larger clumps. RAW264.7 macrophages were co-incubated with UV-inactivated C. albicans for 2 h and fixed with 4% formaldehyde. Macrophages were stained with CellBrite Green, and C. albicans cells were stained with CFW. ( A ) Confocal microscopy images of macrophages co-incubated with wild type, eng1Δ/Δ, fgr41Δ/Δ, fgr41Δ/ΔENG1 oe , or eng1Δ/Δfgr41Δ/Δ strains. Scale bar = 25 µm. ( B–D ) Quantification of panel A. A total of 40 images were analyzed for each strain. C. albicans cells were counted as being phagocytosed if they appeared internal to the macrophage and/or caused a gap in green fluorescence in the macrophage. ( B ) Percentages of macrophages containing each number of C. albicans cells in panel A. n = the total number of macrophages with at least one C. albicans cell inside them. Graphs including macrophages that had not phagocytosed any C. albicans are found in . ( C ) The number of C. albicans cells inside each macrophage for each strain from panel A. Each point represents a macrophage that had phagocytosed at least one C. albicans cell. The number of macrophages represented in each bar corresponds to the n in panel B. **** P <0.0001, *** P < 0.001, by one-way ANOVA, ns = not significant. ( D ) The wild type and eng1Δ/Δ bars from panel C were analyzed in isolation, which revealed a significant difference (* P < 0.05, by Welch’s t -test).

    Techniques Used: Incubation, Staining, Confocal Microscopy, Fluorescence, Isolation



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    Image Search Results


    Cell separation defects lead to phagocytosis of larger clumps. RAW264.7 macrophages were co-incubated with UV-inactivated C. albicans for 2 h and fixed with 4% formaldehyde. Macrophages were stained with CellBrite Green, and C. albicans cells were stained with CFW. ( A ) Confocal microscopy images of macrophages co-incubated with wild type, eng1Δ/Δ, fgr41Δ/Δ, fgr41Δ/ΔENG1 oe , or eng1Δ/Δfgr41Δ/Δ strains. Scale bar = 25 µm. ( B–D ) Quantification of panel A. A total of 40 images were analyzed for each strain. C. albicans cells were counted as being phagocytosed if they appeared internal to the macrophage and/or caused a gap in green fluorescence in the macrophage. ( B ) Percentages of macrophages containing each number of C. albicans cells in panel A. n = the total number of macrophages with at least one C. albicans cell inside them. Graphs including macrophages that had not phagocytosed any C. albicans are found in . ( C ) The number of C. albicans cells inside each macrophage for each strain from panel A. Each point represents a macrophage that had phagocytosed at least one C. albicans cell. The number of macrophages represented in each bar corresponds to the n in panel B. **** P <0.0001, *** P < 0.001, by one-way ANOVA, ns = not significant. ( D ) The wild type and eng1Δ/Δ bars from panel C were analyzed in isolation, which revealed a significant difference (* P < 0.05, by Welch’s t -test).

    Journal: Infection and Immunity

    Article Title: Loss of Fgr41 in Candida albicans attenuates virulence and increases proinflammatory immune responses in a manner that is dependent on β(1,3)-glucan but not dectin-1

    doi: 10.1128/iai.00523-25

    Figure Lengend Snippet: Cell separation defects lead to phagocytosis of larger clumps. RAW264.7 macrophages were co-incubated with UV-inactivated C. albicans for 2 h and fixed with 4% formaldehyde. Macrophages were stained with CellBrite Green, and C. albicans cells were stained with CFW. ( A ) Confocal microscopy images of macrophages co-incubated with wild type, eng1Δ/Δ, fgr41Δ/Δ, fgr41Δ/ΔENG1 oe , or eng1Δ/Δfgr41Δ/Δ strains. Scale bar = 25 µm. ( B–D ) Quantification of panel A. A total of 40 images were analyzed for each strain. C. albicans cells were counted as being phagocytosed if they appeared internal to the macrophage and/or caused a gap in green fluorescence in the macrophage. ( B ) Percentages of macrophages containing each number of C. albicans cells in panel A. n = the total number of macrophages with at least one C. albicans cell inside them. Graphs including macrophages that had not phagocytosed any C. albicans are found in . ( C ) The number of C. albicans cells inside each macrophage for each strain from panel A. Each point represents a macrophage that had phagocytosed at least one C. albicans cell. The number of macrophages represented in each bar corresponds to the n in panel B. **** P <0.0001, *** P < 0.001, by one-way ANOVA, ns = not significant. ( D ) The wild type and eng1Δ/Δ bars from panel C were analyzed in isolation, which revealed a significant difference (* P < 0.05, by Welch’s t -test).

    Article Snippet: CellBrite Green stain was purchased from Biotium (30021).

    Techniques: Incubation, Staining, Confocal Microscopy, Fluorescence, Isolation

    Nano flow cytometry controls. A) Representative controls for EV staining using ExoBrite CTB and ExoBrite True EV Membrane stain. B) Representative controls for CD9-AF647 staining.

    Journal: bioRxiv

    Article Title: GW4869 depletes macrophages and increases number of extracellular vesicles in murine peritoneal cavity fluid

    doi: 10.64898/2026.01.20.700528

    Figure Lengend Snippet: Nano flow cytometry controls. A) Representative controls for EV staining using ExoBrite CTB and ExoBrite True EV Membrane stain. B) Representative controls for CD9-AF647 staining.

    Article Snippet: The next day, samples were stained at 1X concentration with a mix of the membrane dyes ExoBrite 410/450 CTB (#30111-T, Biotium) and ExoBrite 515/540 True EV membrane stain (#30129-T, Biotium) which was diluted in PBS and 50 uL added to the samples and incubated 1-2 hours at RT.

    Techniques: Flow Cytometry, Staining, Membrane